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cdna coding for hla molecules  (Inserm Transfert)

 
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    Inserm Transfert cdna coding for hla molecules
    a Identification of <t>the</t> <t>cDNA</t> regions coding for the B*4002 restricted tyrosinase epitope. Tyrosinase fragments were obtained by exonuclease digestion. Fragments recognized by the specific M88-74 CTL clone are indicated with a positive sign in the right column. The positions are indicated in base pairs. Fragments were co-transfected into COS-7 cells with the appropriate <t>HLA</t> cDNA. Fourty-eight hours after transfection, cells were incubated for 6 h at 37°C with the CTL clone and TNF secreted in the supernatant was measured by assessing its cytotoxicity for WEHI 164 clone 13. b TNF secretion by M88-74 CTL clone in response to COS-7 cells transfected with the B*4002 cDNA and the full-length cDNA coding for tyrosinase and with the tyrosinase fragments 1–933 and 1–975
    Cdna Coding For Hla Molecules, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+coding+for+hla+molecules/pmc11031056-195-35-51?v=Inserm+Transfert
    Average 90 stars, based on 1 article reviews
    cdna coding for hla molecules - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "A new tyrosinase epitope recognized in the HLA-B*4002 context by CTL from melanoma patients"

    Article Title: A new tyrosinase epitope recognized in the HLA-B*4002 context by CTL from melanoma patients

    Journal: Cancer Immunology, Immunotherapy : CII

    doi: 10.1007/s00262-008-0551-0

    a Identification of the cDNA regions coding for the B*4002 restricted tyrosinase epitope. Tyrosinase fragments were obtained by exonuclease digestion. Fragments recognized by the specific M88-74 CTL clone are indicated with a positive sign in the right column. The positions are indicated in base pairs. Fragments were co-transfected into COS-7 cells with the appropriate HLA cDNA. Fourty-eight hours after transfection, cells were incubated for 6 h at 37°C with the CTL clone and TNF secreted in the supernatant was measured by assessing its cytotoxicity for WEHI 164 clone 13. b TNF secretion by M88-74 CTL clone in response to COS-7 cells transfected with the B*4002 cDNA and the full-length cDNA coding for tyrosinase and with the tyrosinase fragments 1–933 and 1–975
    Figure Legend Snippet: a Identification of the cDNA regions coding for the B*4002 restricted tyrosinase epitope. Tyrosinase fragments were obtained by exonuclease digestion. Fragments recognized by the specific M88-74 CTL clone are indicated with a positive sign in the right column. The positions are indicated in base pairs. Fragments were co-transfected into COS-7 cells with the appropriate HLA cDNA. Fourty-eight hours after transfection, cells were incubated for 6 h at 37°C with the CTL clone and TNF secreted in the supernatant was measured by assessing its cytotoxicity for WEHI 164 clone 13. b TNF secretion by M88-74 CTL clone in response to COS-7 cells transfected with the B*4002 cDNA and the full-length cDNA coding for tyrosinase and with the tyrosinase fragments 1–933 and 1–975

    Techniques Used: Transfection, Incubation



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    cdna coding for hla molecules  (Inserm Transfert)
    90
    Inserm Transfert cdna coding for hla molecules
    a Identification of <t>the</t> <t>cDNA</t> regions coding for the B*4002 restricted tyrosinase epitope. Tyrosinase fragments were obtained by exonuclease digestion. Fragments recognized by the specific M88-74 CTL clone are indicated with a positive sign in the right column. The positions are indicated in base pairs. Fragments were co-transfected into COS-7 cells with the appropriate <t>HLA</t> cDNA. Fourty-eight hours after transfection, cells were incubated for 6 h at 37°C with the CTL clone and TNF secreted in the supernatant was measured by assessing its cytotoxicity for WEHI 164 clone 13. b TNF secretion by M88-74 CTL clone in response to COS-7 cells transfected with the B*4002 cDNA and the full-length cDNA coding for tyrosinase and with the tyrosinase fragments 1–933 and 1–975
    Cdna Coding For Hla Molecules, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+coding+for+hla+molecules/pmc11031056-195-35-51?v=Inserm+Transfert
    Average 90 stars, based on 1 article reviews
    cdna coding for hla molecules - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    a Identification of the cDNA regions coding for the B*4002 restricted tyrosinase epitope. Tyrosinase fragments were obtained by exonuclease digestion. Fragments recognized by the specific M88-74 CTL clone are indicated with a positive sign in the right column. The positions are indicated in base pairs. Fragments were co-transfected into COS-7 cells with the appropriate HLA cDNA. Fourty-eight hours after transfection, cells were incubated for 6 h at 37°C with the CTL clone and TNF secreted in the supernatant was measured by assessing its cytotoxicity for WEHI 164 clone 13. b TNF secretion by M88-74 CTL clone in response to COS-7 cells transfected with the B*4002 cDNA and the full-length cDNA coding for tyrosinase and with the tyrosinase fragments 1–933 and 1–975

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: A new tyrosinase epitope recognized in the HLA-B*4002 context by CTL from melanoma patients

    doi: 10.1007/s00262-008-0551-0

    Figure Lengend Snippet: a Identification of the cDNA regions coding for the B*4002 restricted tyrosinase epitope. Tyrosinase fragments were obtained by exonuclease digestion. Fragments recognized by the specific M88-74 CTL clone are indicated with a positive sign in the right column. The positions are indicated in base pairs. Fragments were co-transfected into COS-7 cells with the appropriate HLA cDNA. Fourty-eight hours after transfection, cells were incubated for 6 h at 37°C with the CTL clone and TNF secreted in the supernatant was measured by assessing its cytotoxicity for WEHI 164 clone 13. b TNF secretion by M88-74 CTL clone in response to COS-7 cells transfected with the B*4002 cDNA and the full-length cDNA coding for tyrosinase and with the tyrosinase fragments 1–933 and 1–975

    Article Snippet: MMP-1 MMP-2 MMP-3 37 38 39 40 41 42 43 44 45 46 MMP-7 MMP-9 MMP-14 MAGE-D Eph 2 Her-2neu RAS WT RAS mut Hsp70 Steap Open in a separate window The cDNA coding for HLA molecules were obtained from T. Boon (LICR, Brussels, Belgium) and from E. Houssaint (Unité 463 INSERM, Nantes, France).

    Techniques: Transfection, Incubation